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intestinal permeability  (MedChemExpress)
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MedChemExpress intestinal permeability
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Intestinal Permeability, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell  (MedChemExpress)
99
MedChemExpress cell
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Cell, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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permeability  (Brugger Feinmechanik)
86
Brugger Feinmechanik permeability
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Permeability, supplied by Brugger Feinmechanik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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membrane permeable ca2 chelator bapta am  (MedChemExpress)
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MedChemExpress membrane permeable ca2 chelator bapta am
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Membrane Permeable Ca2 Chelator Bapta Am, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fangchinoline increased phagosomal membrane permeability  (Galectin Therapeutics)
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Galectin Therapeutics fangchinoline increased phagosomal membrane permeability
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Fangchinoline Increased Phagosomal Membrane Permeability, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non permeable substrate  (Avery Dennison)
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Avery Dennison non permeable substrate
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Non Permeable Substrate, supplied by Avery Dennison, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc dextran permeability assay in vitro fluorescein isothiocyanate fitc conjugated dextran 3000 kda mw  (Thermo Fisher)
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Thermo Fisher fitc dextran permeability assay in vitro fluorescein isothiocyanate fitc conjugated dextran 3000 kda mw
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Fitc Dextran Permeability Assay In Vitro Fluorescein Isothiocyanate Fitc Conjugated Dextran 3000 Kda Mw, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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permeability biocompatible synthetic polysulfone membrane  (Fresenius Medical Care)
86
Fresenius Medical Care permeability biocompatible synthetic polysulfone membrane
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Permeability Biocompatible Synthetic Polysulfone Membrane, supplied by Fresenius Medical Care, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caco 2 permeability  (ATCC)
99
ATCC caco 2 permeability
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Caco 2 Permeability, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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permeability  (Hasegawa Co Ltd)
86
Hasegawa Co Ltd permeability
Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on <t>intestinal</t> barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal <t>permeability,</t> as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.
Permeability, supplied by Hasegawa Co Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on intestinal barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal permeability, as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.

Journal: Drug Design, Development and Therapy

Article Title: Probiotics in Combination with C16 Peptide and Angiopoietin-1 Synergistically Ameliorate Multiple Sclerosis in Mice

doi: 10.2147/DDDT.S614294

Figure Lengend Snippet: Protective effects of the C16 and Ang-1 (C+A), and C+A+probiotics treatments on intestinal barrier integrity in EAE mice. ( A – D ) Immunofluorescence staining images of ZO-1 (a specific tight junction marker, green) in the colon, with the cell nuclei stained by Hoechst 33342. ( A ) In normal mice, ZO-1 was obviously expressed on the surface of the intestinal mucosa. ( B ) In the vehicle treated group, there was a decrease in ZO-1 expression. ( C ) The ZO-1 expression increased in the probiotics group, when compared to the vehicle group. ( D ) Probiotics combined with C16+Ang-1 further increased the ZO-1 expression. ( E ) The quantification of ZO-1 expression (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. ( F ) Increased intestinal permeability, as indicated by the FITC-dextran absorption levels, was observed in the vehicle group. Permeability decreased in the probiotics group, and significantly decreased in the C+A+probiotics group. (a) p <0.05 vs the control group; (b) p <0.05 vs the vehicle group; (c) p <0.05 vs the probiotics group. Scale bar = 100 μm.

Article Snippet: Intestinal permeability was evaluated by measuring the flux of fluorescein isothiocyanate (FITC)-Dextran 4000 (4 kDa, CAS:60842–46-8, MedChemExpress).

Techniques: Probiotics, Immunofluorescence, Staining, Marker, Expressing, Control, Permeability